Background: The present study was conducted for assessing alteration of skin barrier function by Staphylococcus aureus-derived lipoteichoic acid (ALA) in atopic dermatitis. Material and methods: For evaluating alteration of skin barrier function by Staphylococcus aureus-derived lipoteichoic acid in atopic dermatitis patients, a total of 20 patients were evaluated. Inclusion criteria for the present study included patients with presence of atopic dermatitis. In-vitro samples were obtained and direct exposure of T cells was done. Fluorescein isothiocyanate contact hypersensitivity models for TH2-mediated cutaneous inflammation. All the results were recorded in Microsoft excel sheet and were subjected to statistical analysis using SPSS software. Results: ALA was found to effectively inhibit the activation of T lymphocytes in a manner that does not depend on Toll-like receptor 2. T cells that were exposed to ALA exhibited neither proliferation nor cytokine production. Consequently, exposure to ALA led to a transient state of functional paralysis in T cells. Furthermore, ALA significantly diminished both T-cell cytokine production and cutaneous recall responses. Conclusion: Improved identification and characterization of atopic dermatitis is required to optimize the precision medicine approach.