Introduction: Fistula-in-ano is a common medical condition affecting about 8.6 people per 100,000 people, resulting from untreated or inadequately drained cryptoglandular abscesses, Crohn’s disease, radiation or carcinoma of the rectum or anal canal. It causes perianal pain, discharge and significant distress and embarrassment to the patient. Although treatment for anal fistulae has many approaches, surgeons have a challenging task dealing with postoperative complications like recurrence and incontinence as they are highly likely with conventional methods of approach. Hence, new sphincter-saving approaches are proposed and analyzed for the treatment of fistulae, amongst which one such method is the laser ablation of the fistula tract, which shows promising results in terms of patient satisfaction and surgical outcome. Methods and Materials: This randomized, controlled study included 68 patients, equally allocated into two groups by random allocation software. All patients were evaluated preoperatively with an MRI fistulogram and mechanical bowel preparation. Group A patients underwent Laser Ablation of Fistula Tract, and Group B patients underwent Fistulectomy. Postoperative patients were analyzed and compared between the two groups in terms of postoperative pain, discharge, duration of hospital stay, recurrence and anal incontinence for six months. Results: The study's results were analyzed using SPSS, and it was found that the median age was 40 years, with a male preponderance. A statistical significance was noted in the duration of hospital stay, postoperative pain, and wound discharge. The postoperative pain score in Group A was 3, whereas in Group B was 5. 47% of the patients in Group B had wound discharge, whereas no cases in Group A had this complaint. The mean operative time is 25 mins in Group A patients and 33 mins in Group B patients. When analyzed in both groups, recurrence showed a similar rate of 17.6%. No major forms of incontinence were reported in the groups. Conclusion: Laser Ablation of the Fistula Tract is a safe, effective and minimally invasive option for the treatment of anal fistulae. The procedure has the added advantage of reduced postoperative pain, wound discharge, and duration of hospitalization with reduced operative time, hence increasing patient satisfaction. Laser Ablation of the Fistula Tract has a short learning curve and can be easily practiced. Furthermore, this procedure can be done multiple times in a patient with minimal risk of sphincter injury.