Background: Drug resistance among Candida species is a serious concern. The present study is undertaken to perform the antifungal susceptibility of the Candida species isolated from the cases of vulvo vaginitis from a tertiary care centre. Methods: High vaginal swabs from 350 patients with symptoms of vulvovaginitis cases were collected and organisms were identified by gram stain, KOH wet mount and culture on Sabourauds Dextrose agar. Species differentiation was done by standard methods. Antifungal susceptibility was performed by disc diffusion methods. Result: A total number of 116 (33.1%) subjects showed pure growth of Candida species by conventional standard methods. Isolation of NAC was higher (26.7%) than C.albicans. The most common species isolated by conventional methods was C. albicans (36.3%) followed by C. glabrata (24.1%), C. tropicalis (22.5%), C. krusei (10.3%) and C.parapsilosis (7.7%). Disc diffusion results showed maximum resistance to ketoconazole (20.6%) followed by that to fluconazole and nystatin (15.5%), amphotericin B (8.6%) and voriconazole (7.7%). Conclusion: Identification of the species and antifungal susceptibility testing is necessary to prevent the spread of drug resistance candida species in the management of vulvovaginal candidiasis.