Background: Corneal collagen cross-linking (CXR) of human collagen is a physiological process to increase the mechanical and chemical stability of corneal. One major disadvantage of the CXR procedure so far is the long total treatment time of 1 hour including a soaking time of 30 minutes for the riboflavin solution and an illumination time of 30 minutes for the UV light. Therefore, to address the patient comfort, and the surgeon’s efficiency, a shorter CXR procedure would be desirable. There are aims to shorten the soaking time by using a different protocol to apply the riboflavin and to shorten the illumination time by increasing the illumination intensity though maintaining the same total applied energy. Treatment time could be shortened to as low as 2 minutes by delivering a higher energy dose of 45Mw/cm2 through various new devices available now. But this accelerated procedure has not been validated yet. Materials and Methods: An experimental in vitro study was carried out on 40 eye bank corneas with 10 corneas as control, without cross linking,10 corneas underwent conventional CXL procedure with exposure to 3 Mw/cm2 of Ultraviolet(UV) light for 30 minutes, 10 corneas exposed to 9 Mw/cm2 of UV light for 10 minutes and 10 corneas exposed to 18 mw/cm2 of UV light for 5 minutes after debridement of cornea and soaking in riboflavin for 30 minutes. A test cell was fabricated to allow a differential pressure in range 15-20 mm Hg to be applied on the back side of test cornea while keeping it immersed in balanced salt solution, and images were acquired with polarizing light and set of lenses and camera. Results and conclusion: The results were in the form of images. The amount of stress decreases and the biomechanical strength increases with increase in irradiance time due to longer effect of UV exposure. The results were inconclusive regarding the effect of various protocols on optical quality of cornea.