Background: this study was conducted to evaluate the role of preoperative antibiotic prophylaxis in prevention of surgical site infections. Material and methods: The chi-squared and Fisher's exact tests were used to collect and analyse the patient's data while keeping its confidentially, and the student's t-test was used to determine whether there was a difference in means. Results: The types of surgical procedures that were performed in Group A patients included 30 laparoscopic cholecystectomies, 30 open appendectomies, and 20 laparoscopic appendectomies. Group B included 20 patients who never received prophylactic and were not on preoperative antibiotics, of whom 10 had open appendectomies, 2 had laparoscopic appendectomies, and 8 had laparoscopic cholecystectomies. Surgical site infection developed in 2 out of 80 patients in Group A (2.5%), which is significantly lower than the number reported in 1 out of 20 patients (5%) in Group B (P≤ 0.05). Conclusion: Preoperative prophylactic antibiotic usage considerably shortens hospital stays and decreases the occurrence of surgical site infections. The recommendations of both national and international guidelines are applicable to a number of antibacterial medications that were used prophylactically in surgical practise.