Introduction: Asthenopia decreases performance and productivity and affects quality of life. Children can experience many of the same symptoms related to computer use as by adults. Extensive viewing of the computer screen can lead to eye discomfort, fatigue, blurred vision and headaches, dry eyes and other symptoms of eyestrain. Hence, the present study was conducted to assess the magnitude of asthenopia and its association with refractive errors in a population of 6-25 years of age presenting at eye OPD in a tertiary care centre. Material and Methods: The present study was conducted among 1100 individuals aged 6-25 years who reported to out-patient department of Ophthalmology, People’s College of Medical Science & Research Centre, Bhopal with symptoms of asthenopia. Baseline demographic data was collected in Proforma. Reports of eyestrain symptoms were sought in questionnaire on asthenopia related symptoms. Patients were first tested for non-cycloplegic auto-refraction, uncorrected visual acuity (UCVA) with help of Snellens chart, retinoscopy was done using a streak retinoscope and orientation of Streak for astigmatism was done. Results: A total of 1100 patients were screened, and of them 750 (68.2%) cases had asthenopia. 1500 eyes of 750 cases were analyzed and most common cause of asthenopia was refractive error (83.0%), followed by muscular weakness (36.2%). Headache was the most common clinical feature noted in 44.5% cases with asthenopia,followed by watering in 30.4% and blurring in 24.4% cases. Irritation and eye strain were associated features in 18.9% and 19.9% cases respectively. Conclusion: Thus, to conclude our study, we found that asthenopia is present in a significant portion of young population, refractive error being a major contributing factor besides muscular weakness, computer vision syndrome, dry eye. Moreover, until recently little information is available regarding the asthenopia with risk factors among children and college students. The decrease in asthenopia and morbidities associated with it among students (6-25 years of age) can lead to an important benefit to promote next generation of their career and health, both intellectually and psychologically, since these are the formative years in life, hence, justifying the need for research in this area.