Introduction: ‘Sepsis is defined as the systemic inflammatory response syndrome (SIRS) in the context of a suspected or confirmed infection, according to the international paediatric sepsis consensus conference.’ CD64 is a leukocyte surface antigen that is expressed at low levels on nonactivated neutrophils' surfaces. The Fc receptor, which is increased during infection and sepsis, has a high affinity for CD64. In the event of bacterial infection, CD64 expression in preterm and term new born is comparable to that in older children and adults. Furthermore, CD64 expression is constant at room temperature for more than 30 hours, in contrast to CD11b and other PMN antigens, which are labile. Paediatric sepsis is rarely discussed as a primary cause of death in under developing nations. Early onset sepsis can be challenging because of overlapping clinical manifestations of a range of non-infectious illness such as aspiration syndrome, maladaptation & respiratory distress syndrome. Materials and Methods: This cross sectional study was conducted in Paediatric Emergency Ward, Neonatal and Paediatric Intensive Care Unit, KLES Dr. Prabhakar Kore Hospital and Medical Research Centre, Belgaum, a teaching hospital affiliated with Jawaharlal Nehru Medical College, Belagavi from January 2020 to August 2021. Patients clinically suspected of having sepsis admitted to Paediatric Emergency Ward, Neonatal and Paediatrics Intensive Care Unit, Belagavi. All cases of clinically suspected sepsis admitted in Emergency paediatrics ward, NICU & PICU were included in the study. Result: Results shows that majority of the cases were in the age group of less than 1 month (64%), followed by 18% in 1 to 5 years.56% of patients were females and 44% were males. The female to male ratio was 1.27.out of total 50 cases, 46 cases show deranged white blood cell count in which 38 cases were blood culture positive and 8 cases were negative.27 cases show deranged absolute neutrophil count in which 21 cases were blood culture positive and 6 cases were negative. The general sensitivity of all the septic screenings is good, as can be observed. Sensitivity has been claimed to be as high.