There are many controversies regarding strengthening exercise for knee pain because until now there is a trend of quadriceps strengthening more than hamstring. The purpose of this randomized controlled experiment was to examine the efficacy of quadriceps and hamstring strengthening activities in the treatment of non-specific knee pain. Thirty people with generalized knee discomfort were randomly allocated to either the quadriceps or hamstring strengthening groups. The exercise program was followed for six weeks, with five sessions each week. Pain intensity was assessed using the Visual Analogue Scale (VAS) before and after the intervention. Both exercise regimens tested for knee discomfort, however hamstring strengthening activities were promising and result oriented than quadriceps strengthening exercises. These findings highlight the significance of specific muscle group workouts in the treatment of knee discomfort.