Background: Laparoscopic cholecystectomy (LC) has become the standard treatment of benign gallbladder diseases. The present study shows alterations in serum bilirubin levels pre and post laparoscopic cholecystectomy. Materials &Methods: A total of 100 patients scheduled to undergo LC were enrolled. Detailed demographic information for all patients was collected. Blood samples were taken to measure serum bilirubin levels before the surgery (baseline). The analysis was performed using SPSS software. Results: We measured the mean levels of total serum bilirubin at three different time points: preoperative, 24 hours postoperative, and 72 hours postoperative. The mean levels at these time points were found to be 0.80 mg/dL, 1.62 mg/dL, and 0.78 mg/dL, respectively. Conclusion: Serum bilirubin levels are significantly altered among patients undergoing Laparoscopic cholecystectomy.