Objective- To assess genomic (DNA and chromosomal) damage in judo players. Design- The study was approved by Institutional Ethics Committee. The players for the present study were contacted from the university during their training. Setting- The blood and buccal samples were collected in the morning session before the warm-up and the start of their training session. For DNA and chromosomal damage assessment, the Single Cell Gel Electrophoresis (SCGE) and buccal micronucleus (MN) cytome assays were performed respectively. Participants- Volunteers (n=27) included judo players competing at inter-university and state levels and age- and sexmatched healthy controls. Results- Highly significant DNA damage in blood leucocytes as well as chromosomal damage (cytokinetic defect and cell death) in buccal epithelial cells of Judo players was observed. No gender differences were found. Significant associations were observed between some genetic damage parameters and sport related variables (warm-up time and time/years of exercising). Conclusion- The genetic injury/damage in different cells of the players requires intervention and management gives the tendency for it to cause malignancy, age related diseases and neurological disorders.