Introduction: Urinary tract infection is one of the most commonly encountered genitourinary disease in paediatric practice. Diagnosis and management of urinary tract infection is a matter of concern in hospital settings and at community level. It accounts for significant morbidity due to potentially dangerous sequelae like renal scarring and hence warrants an early and accurate diagnosis. The clinical diagnosis of urinary tract infection is difficult, due to non- specific or vague symptomatic spectrum seen in children. Often, clinical diagnosis needs to be supported with confirmatory tests like urine culture, which guides in treatment of the infection. Objective: To determine the proportion of bacteriological profile diagnosed by urine culture. Methodology: Study Design: A Cross-sectional study. Study Population:Children between 2 to 12 years of age attending the Paediatric OPD presenting with clinical features of Urinary tract infection. Sample size:75. Sampling Technique: Purposive sampling. Study Period: November 2019 to November 2020.Study procedure: The urine collection technique was explained by the principal investigator in the OPD to the parents as well as the child in the form of video demonstration. Informed consent & assent was obtained. Urine samples was obtained under strict aseptic precautions for urine culture in a sterile container. The sample was then sent to microbiology lab for urine culture. The results obtained from urine culture were analyzed. Data analysis:Data was entered into MS Excel and was analyzed using the SPSS trial version software. The type of bacteria also was analyzed as percentage. Results: Of the total 75 total samples, 33 were urine culture positive. From the 33 culture positive samples, E. coli was most common isolated organism in 25 children (33.3%), followed by Klebsiella pneumoniae in 4 children (5.3%), Proteus in 2 (2.7%), Enterobacter spp. and Pseudomonas in 1 each. Conclusion: The current study shows E. coli to be the most common pathogen in UTI. This warrants the careful selection and judicious use of antibiotics.