Background: Menopause is defined by W.H.O. as “thefinalmenstrualperiod.” This is itself a status of oxidative stress in women’s life which needs good care in this time period. Objective: To estimate the levels of malondialdehyde (MDA), Vitamin C, Uric acid, Albumin and Estrogen in pre and postmenopausal women. Materials and Methods: Total hundred female subjects including fifty healthy premenopausal women as controls and fifty healthy postmenopausal women as cases were enrolled for the study. Five ml of venous blood was drawn under aseptic precautions from antecubital vein in red capped evacuated blood collection tube. Samples were collected after overnight fast of 10–12 hours. Samples were processed within one hour of collection. Serum was separated by centrifugation at 2000 RPM for 10 minutes after clotting. Separated serum was stored at -20°C if not analysed on the same day. Inclusion criteria for cases and controls1) Healthy premenopausal (twenty five to forty years) women without any menstrual irregularities. 2) Healthy postmenopausal women with history of natural menopause (three to five years of menopause). Results: The levels of vitamin C were found to be significantly lower in cases than controls (p