Background: Vitamin D is important for maintaining bone health, immune function, and cardiovascular health. Vitamin D deficiency is prevalent worldwide and has been associated with an increased risk of several chronic diseases, including dyslipidemia, a condition characterized by an abnormal lipid profile that is a major risk factor for cardiovascular diseases. The relationship between vitamin D status and dyslipidemia is not fully understood, and previous studies have reported inconsistent results. The present study aims to investigate the correlation between 25(OH)D and serum lipid profile among asymptomatic adults to provide insights into the relationship between vitamin D status and dyslipidemia, which may aid in the development of preventive strategies and personalized treatment plans for dyslipidemia and CVD. Methods: This study was a cross-sectional observational study conducted on asymptomatic adults between December 2021 and November 2022. Participants were enrolled using convenient sampling, and were divided into two groups based on their level of Vitamin D. Demographic details were noted, and blood samples were collected to measure serum 25-hydroxyvitamin D [25(OH)D] and lipid profile. Descriptive statistics and statistical tests, such as the chi-square test and unpaired t-test, were used for data analysis. Pearson's correlation coefficient was used to determine the correlation between serum 25(OH)D levels and serum lipid profile. A p-value less than 0.05 was considered statistically significant. Results: Of the participants, 55.3% were male and 44.7% were female. The mean age of the participants was 39.12±8.91 years and the mean BMI was 26.54±5.43 kg/m².Of the 179 participants, 83 (46.4%) were deficient, 74 (41.3%) were insufficient, and 22 (12.3%) had sufficient Vitamin D levels.The mean values of total cholesterol (TC), triglycerides (TG), and very low-density lipoprotein (VLDL) were significantly higher in the deficient group compared to the insufficient and sufficient groups (p=0.03).There is a significant negative correlation between Vitamin D levels and Total Cholesterol, Triglyceride, Very Low-Density Lipoprotein, and Low-Density Lipoprotein levels among study participants. Conclusion: Present study provides evidence that a large proportion of adults have Vitamin D deficiency or insufficiency and that this is associated with adverse lipid profile parameters. The findings of this study highlight the importance of Vitamin D screening and supplementation for individuals with Vitamin D deficiency or insufficiency, particularly those with dyslipidemia or other risk factors for cardiovascular diseases.