The prevalence of low back pain ranges from 15 to 30 %. Approximately 80% of the population will experience low back pain at least once in their lifetime. Paraspinal muscles play an important role in the stability and functional movement of the lumbar vertebral column. Recent studies have suggested that patients with low back pain have increased muscle fat infiltration and reduced muscle cross sectional area of paraspinal muscles (multifidus, erector spinae, and psoas muscle) than asymptomatic control patients. Adult patients of either sex with low back pain due to degenerative lumbar spinal stenosis with or without neurological deficits visiting or admitted to our hospital from February 2021 to October 2022 were taken into the study. A total of 40 patients are included in this cross-sectional study. Quantitative measurements of multifidus, erector spinae, and psoas muscles were obtained from 1.5 Tesla MRI machine. All muscle measurements were taken bilaterally at the level of the superior endplate of the L5 vertebra and the inferior endplate of the L5 vertebra. There is a statistically significant association between AP canal diameter at the inferior endplate, Multifidus muscle fat infiltration at the superior and inferior endplate of the L5 vertebra, Multifidus RCSA at the superior endplate of the L5 vertebra with ODI score in univariate analysis. There is no statistically significant multivariate association between the paraspinal muscle morphology and low back pain disability measured by ODI score.