Background: Cervical cancer is the fourth most prevalent cancer in women and eighth most common cancer worldwide, with especially high incidence in developing countries. According to the recommendations of the World Health Organization, Human Papilloma Virus vaccines are effective prevention for cervical cancer caused by carcinogenic HPV infection. Objective: To assess knowledge & awareness among students & employee in a tertiary care centre about HPV infection and vaccination. Methods: A cross-sectional survey was targeted to conduct among 300+ health care professionals including medical, paramedical, nursing students, staff and employee among 18 to 45 years of age group. Subjects were gender neutral and participated vountarily in a tertiary care centre, during the period of study for 3months. Results: We assessed knowledge regarding HPV infection/cervical cancer and HPV vaccine knowledge, awareness and vaccination status. The level of HPV knowledge was found to be low, but more than 80% of participants were ready to accept and recommend HPV vaccine. Conclusion: In India, where the disease burden is high, patient education for primary screening and HPV vaccination is critical, which can only be accomplished if healthcare providers are well-versed in all areas of cervical cancer prevention and management.The purpose of this study was to investigate the knowledge about HPV vaccines and willingness to accept or recommend HPV vaccination among medical staff, students, and employees as they constitute the most visible frontline personnel providing health education to patients and general population .So a validated questionnaire served as a useful tool in the primary prevention setting.