Aim: Analysis of age-related changes in the microanatomy of the ascending aorta and the pulmonary trunk. Materials and Methods: Throughout the course of the autopsies that were performed in the anatomy department, the ascending aorta and the pulmonary trunks were removed. There were a total of 50 samples taken, four of which belonged to the fetal group (one of 32 weeks and another of 34 weeks). All of the samples were taken during the first six hours after the subject's death to prevent any autolytic alterations. Results: There was a lack of subendothelial connective tissue in group I (the fetal group) in both the AA and PT studies. It was found between the internal elastic lamina (IEL) and the endothelium beginning in group II and continuing forward. With increasing age, there was a continuous rise in the thickness of tunica intima of both the vessels, and statistically speaking, this was a significant finding (p 0.001). The average intimal thickness for AA ranged from 5.77 to 271.52 microns, with group I having 5.77 microns and group IV having 271.52 microns. When it came to PT, the average thickness grew from 2.11 to 150.25 microns from group I to group V. In the case of PT, the 20-40 range had the largest average complete wall thickness. It was 839µ. While there were variations in complete wall thickness that were statistically significant across various age groups (p 0.001), the comparison of people with ages less than or equal to 60 years and those older than 60 years did not indicate any statistical significance (p = 0.36). Conclusion: Both of the large vessels exhibited signs of degeneration as they became older; however, during the first decade of life, the alterations were far less severe in the PT than they were in the AA.