The spleen is the largest of the secondary lymphoid organ, plays a vital role in hematopoiesis and immunomodulation of the fetus. Splenic shape and size varies according to the age and weight. The aim of the study is to study variations on morphology and morphometry of human fetal spleen at different gestational ages. The present study was carried out on 100 aborted preserved human fetuses of four different gestational age groups ranging from 11-30 weeks in the department of Anatomy, Government Medical College, 32 Chandigarh. The fetuses were classified into 4 groups. The length of superior, intermediate and inferior borders and width of the spleen were measured and correlated with gestational age. The average length of superior border was found to be 6.68±1.95 mm in group I, 10.55±3.34 mm in Group II, 14.76±2.90 in Group III and 17.68± 3.63mm in Group IV respectively. The mean length of intermediate border was 5.92±1.65 mm in group I, 9.41±2.72 mm in Group II, 12.96±2.42 in Group III and 16.39± 3.95 mm in Group IV respectively and inferior border was 6.15±1.94 mm in group I, 9.78±2.86 mm in Group II, 13.79±3.28 in Group III and 16.48± 3.92mm in Group IV while the mean width of spleen was 7.78±3.59mm.On an average the mean length and breadth of the fetal spleen in different gestational age groups showed an increasing trend and they presented statistically significant values (p=0.001) on comparison with increasing gestational age. These values of fetal splenic size could provide a useful diagnostic tool in the detection of congenital splenic abnormalities and can be used as an indicator of fetal growth.