Introduction: Breast cancer is the most common cancer in women in India and accounts for 14% of all cancers in women. Its survival rate is very low due to presentation of women at advance stage of the disease. Aims and Objective: To assess the knowledge, attitude and practice regarding risk factors, symptoms and screening methods of breast cancer in 40-65 years females and to assess the determinants associated with KAP. Material and Methods: This community based cross sectional study was conducted among 545 females of 40-65 years of age group in villages of Mulugu Mandal, District, Siddipet, Telangana and their KAP was assessed. Results: Mean age of the study participants was 52.10±8.72. 98.5% were married women out of which 265(48.6%) married at the age of 15 years. Hindus were 84.4% by religion. Women with 1st-5th class education was 34.1% and illiterate 27.3% followed by 6th-10th and inter and above i.e., 23.5% and 15%, respectively. Lower middle class were 32.5% followed by middle, lower class, upper middle and upper class are with 31.9%, 20%, 10.1% and 5.5% respectively. Women with good knowledge on risk factors was 290 (53.2%), symptoms 294(53.9%), self-breast examination was 141(25.8%) and mammography was 149(27.4%). Females with positive attitude towards self-breast examination and mammography was 141(25%) & 149 (27%). Total number of women who practice self-breast examination regularly was only 35 (6.4%) whereas mammography was 45(8.2%). Conclusion: Knowledge on risk factors, symptoms and screening methods for breast cancer is low, and practice of self-breast examination and mammography is very low.