Background: Disabling Hearing Loss is defined as hearing loss greater than 40 dB in the better hearing ear in adults (15 years or older) and greater than 30 dB in the better hearing ear in children (0–14 years). Otoacoustic emission tests (OAE) and automated auditory brainstem response tests (BERA) are the most effective two categories of objective screening tests. Both tests have a high sensitivity and specificity for detecting Hearing loss that is worse than 40 dB. Objective: Early detection of hearing loss in infants and to know the prevalence of hearing loss in infants and compare the hearing status of normal and preterm infant. Methodology: A Prospective observational study was in Bundelkhand Medical College, Sagar, M.P. conducted on 125 infants out of which 75 were premature and 50 were normal over a period of one year (Oct 2021 to Oct 2022). The infants were selected on the basis of inclusion and exclusion criteria, then informed consent was taken from the parents and all infants were categorized as premature infants and normal infants with the help of birth history and clinical examination. Screening was done with Otoacoustic Emission technique on two occasions (First visit within 6 months of age and Second visit after 1 month of first visit )and results were analysed. Result: The level of prematurity in total 19 infants : 16 out of 75 premature infants (21.3%) in comparison to 3 full-term infants out of 50 (6%) had abnormal hearing response on OAE. The distribution of abnormal Hearing and degree of prematurity was statistically significant (p-value = 0.046). [36% were very premature, 47% were moderately premature, and remaining 15% were full term. Among 19 newborns with abnormal OAE, there was history of admission to NICU. Conclusion: Hearing loss is a serious complication of premature birth, and its incidence declines as the foetus matures in the mother‘s womb.