Background: Lipid profile alteration is a useful indicator for early warning of the severity of various cardiovascular diseases. This study was performed to assess the effects of Covid 19 related lockdown on atherogenic lipid profiles of healthcare workers. Material and methods: A prospective study was carried out on 40 HCWs including 23 male and 17 females between the age of 22 and 59 years. The study was conducted in the department of Biochemistry and the department of Physiology, Government Medical College, Kathua, UT of Jammu and Kashmir. Informed written consent was obtained from the subjects and ethical clearance was obtained from Institutional Ethics Committee. Their fasting blood was collected to estimate their lipid profile which included TG, total cholesterol (TC) and HDL-C. Results: The present study found statistically significant increase in mean HDL-Cholesterol before and after covid-19 pandemic in health care workers. Conclusion: The results of this study demonstrated that lockdown had a substantial impact on participants' lipid profiles as their lifestyles changed during pandemic.