Objective: Tuberculosis bacilli are constantly evolving, enabling them to efficiently adapt to new environments and gain multidrug resistance. As a result, treatment of drug resistant tuberculosis is becoming a challenge. Young medical graduates are the future of healthcare workforce in a country. The present study was undertaken with objective to study the Knowledge, Attitude and Practice associated with multidrug resistance in TB among Medical interns & postgraduates at M. R. Medical College, Kalaburagi. Materials and Methods: This was a cross-sectional survey conducted by means of pre designed questionnaire. 81 medical interns, 82 postgraduates were interviewed for socio-demographic information and questions about knowledge, attitude and practices regarding multidrug resistance in TB. Results: Out of the 163 participants, response rate was 92%. The participants had sound knowledge about MDR TB, but 43% (n=64) were unaware about recent updates in treatment out of which 21 were interns. All participants showed positive attitude towards attending TB training programs. Mere 71% participants practiced using protective masks while attending patients. Few participants (38%) were counseling patients regarding MDR TB and mere (39%) were sending sample for drug sensitivity testing in resistant cases.Conclusion: Our study provides an overview of the knowledge, attitudes and practices regarding MDR TB. Survey revealed that most of the participants were clear about the concept of MDR TB. The matter of concern was poor awareness about recent updates in RNTCP guidelines. The study emphasized need for continuous educational interventions necessary to upgrade their understanding, recent advances, as well as change their attitude towards patients.