With respect to the realization that hand hygiene is a prerequisite for the prevention of diseases, the conventional method of washing hand with soap is not quite popular these days. Instead it is the use of handsanitizer, which has gradually become the method of choice due to its various advantages. In the present work, alcoholic and nonalcoholic hand sanitizers were prepared in the form of polymer based gels and their antiseptic efficacy was evaluated. Microbiological methods revealed that alcoholic hand sanitizers were more efficacious in providing hand hygiene.