Background: For patients receiving persistent haemodialysis, maintaining effective vascular access is a critical concern. The preferred vascular access for hemodialysis is an arteriovenous fistula (AVF) made from native veins. At equivalent flow rates, the AVF is linked to a lower incidence of problems and longer survival than prosthetic grafts or central venous catheters. Thus, the purpose of the current study was to evaluate the efficiency of colour Doppler ultrasound (CDUS) in the treatment of arterio-venous fistulas associated with dysfunctional hemodialysis. Materials &Methods: The goal of the current study was to evaluate the efficacy of color Doppler ultrasound (CDUS) in treating arterio-venous fistulas associated with dysfunctional hemodialysis. Twenty patients in all were enrolled. Prior to US inspection, each patient had their dialysis access evaluated clinically. The patient is often positioned supine, with the arm relaxed and extended out to the side, with the area being assessed closest to the sonographer, for DU exams. Arterio-venous fistula in a dysfunctional hemodialysis patient using color Doppler ultrasonography (CDUS). Additionally performed for evaluation was digital subtraction angiography. SPSS software was used to record and evaluate each outcome. Results: The stenotic region has a 1.5 mean diameter. The mean prestenotic and interstenotic velocities were 71.5 and 246.2, respectively. Comparing CDUS to DSA (digital subtraction angiography), the sensitivity and specificity of CDUS for the detection of substantial stenosed vessel segments were 95.6 percent and 97.2 percent, respectively.Conclusion: The monitoring of the blood flow through the AVF and the identification of potential reasons for vascular access dysfunction are both made feasible by CDUS, a non-invasive diagnostic tool for early diagnosis of problems of AVFs.