A field experiment was conducted at CCS Haryana Agriculture University, Hisar Haryana during 2005-06 and 2006-07 to study the effect of organic sources of nutrition and irrigation levels on yield, nutrient uptake and nutrient use efficiency of wheat.The grain yield increased by 19.4 and 22.6 % in I2 over I1 and 12.5 and 14.2 % over I2 in I3 (3868 and 4060 kg/ha) during 2005-06 and 2006-07, respectively. The increase in irrigation frequency increased the N (18.8 and 23.6 %), P (19.7 and 23.4 %) and K (17.4 and 16.8 %) uptake over I1 and N (6.0 and 6.7 %), P (5.5 and 6.9 %) and K (5.8 and 4.9 %) uptake over I2 in I3 treatment during two years of study, respectively. The apparent recovery of N also increased by 28.8 and 32.2 % over I1 and 6.5, 16.8 % over I2 in I3 treatment during both the year of experimentation, respectively. The increase in dose of organic manure from 75 to 150 and 225 kg N/ha by any sources increased the grain yield of wheat significantly increased during both the year. The increase in level of poultry manure increased the N, P and K uptake from T9 (80.5, 83.2, 18.9, 19.5, 81.0 and 81.7 kg/ha) and form T10 (93.2, 96.6, 21.6, 22.6, 89.0 and 90.4 kg/ha) in T11 treatment during both the years of study, respectively.