Background: A major global cause of morbidity and death, coronary artery disease (CAD) is impacted by both environmental and genetic factors. One important immune response regulator that has been linked to CAD susceptibility is the CD14 gene. In a case-control population, this study examines the relationship between CAD and a polymorphism in the CD14 gene. Methods: The CD14 gene polymorphism was genotyped in 90 participants, including both healthy controls and CAD patients. To evaluate the risk association, genotype and allele frequencies were examined, and odds ratios (OR) were computed. Results: In CAD cases, the frequencies of CD14 genotypes CC, CT, and TT were 24.44%, 51.11%, and 24.44%, respectively, while in controls, they were 15.55%, 40%, and 45.44%. The ORs for the genotypes CC, CT, and TT were, respectively, 0.57 (P = 0.292), 0.64 (P = 0.29), and 2.47 (P = 0.046). CAD cases had 100% and 75.44% frequencies of the C and T alleles, while controls had 71.1% and 84.44% frequencies, respectively. With an OR of 0.55 (P = 0.05), the C allele provided protection, whereas the T allele was linked to a higher risk of CAD (OR of 1.81).Conclusion: There is a strong correlation between the CD14 gene polymorphism and CAD. While the C allele seems to be protective, the TT genotype and T allele are linked to an increased risk of CAD. These results suggest that CD14 polymorphism may play a part in the pathophysiology of CAD and call for more research to determine its potential therapeutic implications.