Background: This study investigates the forensic role of antidotes in poisoning cases, emphasizing their effectiveness and impact on patient outcomes. Methods: A prospective observational study was conducted over 11 months at Hi-Tech Medical College and Hospital, Bhubaneswar, involving approximately 100 patients presenting with various types of poisoning. Inclusion and exclusion criteria were applied to ensure relevant data collection. Results: Of the 100 patients, 85 (85%) achieved successful recovery following antidote administration. The study revealed that timely administration of antidotes significantly correlated with improved outcomes; 50% of patients received treatment within one hour of exposure. The most common types of poisoning involved pharmaceuticals (45%) and pesticides (30%). Statistical analysis indicated a significant association (p < 0.01) between antidote administration timing and patient recovery. Conclusion: The findings underscore the critical importance of timely antidote administration in enhancing recovery rates in poisoning cases. This study contributes to the understanding of antidotes in forensic toxicology, advocating for rapid assessment and intervention in clinical practice.