Background: Among the many conditions that can affect the gallbladder are congenital defects, calculi and their aftereffects, non-inflammatory, inflammatory, and malignant tumors. The present study was conducted to evaluate CA19-9 and CA 125 level in carcinoma gall bladder. Materials & Methods: 52 cases of carcinoma gall bladder of both genders were assessed for CA19-9 and CA 125 by chemiluminescent microparticle immunoassay (CMIA) method. Results: Out of 52 patients, 32 were males and 20 were females. Lesions were chronic cholecystitis in 28, xanthogranulomatous cholecystitis in 19 and chronic cholecystitis with gastric metaplasia in 5 cases. The difference was significant (P< 0.05). The mean CA19-9 level was 134.2± 7.5 U/ml and CA 125 level was 63.7± 8.2 U/ml. Conclusion: Elevated level of CA19-9 and CA 125 in carcinoma gall bladder was found. These are fairly good markers for discriminating patients of carcinoma of the gallbladder from cholelithiasis.