Background: Chronic suppurative otitis media (CSOM) is characterized by prolonged inflammation of middle ear mucosa and mastoid leading to TM perforation and ear discharge. CSOM is now supposed to be biofilm-related. In biofilms, bacteria are embedded in a slim-like extracellular matrix composed of proteins, polysaccharides and nucleic acids called extracellular polymeric substances.Objectives: This study aimed to determine the incidence of biofilms in bacterial isolates of CSOM cases, bacteriological pattern and their antibiotic susceptibility in biofilm-producing organisms in CSOM, Resistant pattern to antimicrobials among biofilm-producing strains leading to treatment failure and the outcome of early surgical intervention in such patients.Methodology: This prospective study was conducted in the Department ofOtorhinolaryngology in association with the Department of Microbiology. Ear discharge sample is collected using aseptic precautions and culture sensitivity and tests to check for presence of biofilm is done and Early surgical intervention of the patients with biofilms positive were performed. They were evaluated for the discharge-free period, graft uptake and infection-free period during follow-up.Results:Out of 170 patients,155 patients were biofilm positive , the most common organism isolated being Methicillin resistant staphylococcus aureus and early surgical intervention was performed . Postoperative graft uptake is seen in 142(83.5%) patients. Conclusion:Early surgical intervention in biofilm positive csom patients would be helpful in high chances of graft uptake, and in regaining infection free ear