Background: Self medication is extensively prevalent and practiced among students perceiving careers in medical or health care and studying pharmacology might further influence this practice. Objectives: To determine the knowledge, attitude and practice of self medication among Phase II MBBS batch 2022 and batch 2023 medical students and to compare the impact of studying pharmacology on self medication among them. Methods: A cross-sectional, comparative, questionnaire-based study was conducted among Phase II MBBS students of batch 2022 and batch 2023 of Adesh Medical College & Hospital, Haryana. The data was analyzed and the results were expressed as frequency and percentages. Results: Out of 150 responders of Phase II MBBS batch 2022, 56 [37.33%] of 58 male and 86 [57.33%] of 92 female and out of 138 responders of Phase II MBBS batch 2023, 52 [37.68%] of 59 male and 76 [56.07%] of 79 female has taken self medication in last 3 months. Among students of Phase II MBBS batch 2022, 113 [79.57%] had knowledge about adverse drug reactions, 105 [73.94 %] had knowledge about storage of drugs, 121 [85.21 %] had knowledge about generic and brand names, 78 [54.92 %] had knowledge about rational use of antibiotics and 60 [42.25 %] had knowledge about use of drugs in emergency. Among students of Phase II MBBS batch 2023, 21 [16.40%] had knowledge about adverse drug reactions, 48 [37.50 %] had knowledge about storage of drugs, 79 [61.71 %] had knowledge about generic and brand names, 14 [10.93 %] had knowledge about rational use of antibiotics and 15 [11.71 %] had knowledge about use of drugs in emergency. Conclusion: The present study showed the positive impact of studying pharmacology on knowledge, attitude and practice of self medication among medical students.