Background: The present study was undertaken for assessing the serum magnesium levels in diabetic patients.Materials & methods: A total of 50 diabetic subjects were enrolled. Another set of 50 subjects were included as healthy controls. Complete demographic and clinical details of all the subjects was obtained. Blood samples were obtained and serum magnesium levels were evaluated by using auto-analyser. All the results were recorded in Microsoft excel sheet and were analysed using SPSS software. Results: Mean serum magnesium levels among subjects of diabetic group and control group was 1.95 mg/dL and 2.39 mg/dL respectively (p-value < 0.00.) Significant results were obtained while correlating serum magnesium levels with glycemic profile. Hypomagnesia was seen in 36 percent of the subjects. Conclusion: A significant decrease in the serum magnesium levels in patients with Type 2 DM was seen; hence it is important to insist magnesium supplementation on uncontrolled Type 2 diabetes.