Background: The study was conducted for Forensic Analysis of Human Microbiome in Skin and Body Fluids. Material and methods: The present study was conducted for Forensic Analysis of Human Microbiome in Skin and Body Fluids. A total of 100 subjects were evaluated. Inclusion criteria for the present study included subjects who came for routine checkup. Complete demographic and medical details of all the patients was obtained. A Performa was made and complete clinical details of all the patients was obtained. Skin and body fluid samples were obtained and were cultured. Predominant bacterial organism was isolated and recorded. All the results were subjected to statistical analysis using SPSS software. Results: A total of 100 subjects were evaluated. Mean age of the subjects was 51.8 years. Majority proportion were males. In the skin samples, dominant bacteria were Corynebacterium, Staphylococcus and Bacillus. In the saliva sample, dominant bacteria were Streptococcus, Veillonella, Prevotella, Porphyromonas while in the genital swab, Bifidobacterium was the dominant bacteria. Significant results were obtained while correlating bacterial profile among males and females. Conclusion: Skin and body fluids could be employed in assisting forensic analysis.