Background: Several biological factors such as bacterial infections and immunological status are implicated in predisposing individuals to bladder cancer. Bacterial infection of urinary tract has been related to increase the risk of bladder cancer. Patients and Methods: On CLED agar, urine culture will be carried out. At 24 and 48 hours, all plates will be read after being incubated at 37 degree Celsius. Results: Bacterial growth was observed in 15 urine samples of those patients represent (27.2 %) while, 40(72.72%) yielded no growth (negative results). It is obvious that E. coli is the most predominant organisms followed by K. pneumoniae and Ps. Aeruginosa. Conclusion: There is predominance of gram negative, facultative rod shaped anaerobes in the urine of carcinoma bladder cases included in the study