Aim:This study aims to investigate the impact of circadian rhythm disruption on cardiovascular and metabolic health in shift workers, focusing on differences in autonomic function, hormonal balance, inflammatory markers, vascular health, and sleep quality between shift and non-shift workers.Material and Methods:A total of 100 participants, aged 25-55, were recruited and divided into two groups: shift workers (SW) working night or rotating shifts (n=50) and non-shift workers (NSW) working standard hours (n=50). Data were collected at baseline, 3, and 6 months, evaluating blood pressure, heart rate (HR), heart rate variability (HRV), cortisol, lipid profiles, fasting glucose, inflammatory markers (CRP, IL-6), vascular health (flow-mediated dilation and pulse wave velocity), sleep quality, and stress levels. Repeated measures ANOVA and independent t-tests were used for statistical analysis.Results:Shift workers showed increased autonomic dysfunction, with significant reductions in HRV (p=0.0007) and elevated LF/HF ratios (p=0.000002), indicating sympathetic dominance. SW participants also displayed significant reductions in morning cortisol (p=0.0318) and higher inflammatory markers, including IL-6 (p=0.0001) and CRP (p=0.0499). Metabolic markers, particularly HOMA-IR, increased significantly in both groups, but to a higher extent in SW (p=0.00004). Vascular health parameters remained stable across both groups. However, daytime sleepiness and perceived stress increased significantly, particularly in SW participants.Conclusion:Circadian rhythm disruption due to shift work is associated with autonomic imbalance, increased inflammation, and a higher risk of metabolic dysfunction. These findings emphasize the need for interventions targeting circadian realignment and lifestyle changes to improve the cardiovascular health of shift workers.