Background: Endodontic files and irrigation solutions are used in chemomechanical preparation to clean and shape the root canal system. The present study was conducted to assess effects of different glide path files on apical debris extrusion in curved root canals. Materials & Methods: 30 recently extracted mandibular first molar teeth with curved mesial roots were divided into 6 groups according to the root canal preparation (n = 5): group IV-File, a glide path with G-Files + WaveOne Gold preparation; group I, a glide path with One G+ WaveOne Gold preparation; group II, a glide path with ProGlider+ WaveOne Gold preparation; group III, a glide path with PathFiles+ WaveOne Gold; group V, a glide path with a K-file + WaveOne Gold preparation; and group without a glide path, WaveOne Gold preparation without a glide path file. Roots were attached to pre-weighed Eppendorf tubes. Apically extruded debris was collected in Eppendorf tubes. Results: The mean apical debris extrusion was 0.000081 grams in group I, 0.000241 grams in group II, 0.000185 grams in group III, 0.000154 grams in group IV, 0.000323 grams in group V and 0.000192 grams in group VI. The difference was significant (P< 0.05). Conclusion: More debris was extruded by manual K-files than by One G rotary glide path files. Apical debris extrusion was unaffected by glide path preparation prior to root canal preparation with a WaveOne Gold file.