Title: Prevalence of dry eye disease (DED) in rural population of district: Shahjahanpur, Uttar Pradesh, India: A prospective study. Material& methods: A prospective study was conducted from 01/04/2022 to 31/03/2024 for two years. The entire rural population of specific area (block wise) having 272500 persons were contacted. 76196 persons were found symptomatic and were subjected to standard Shirmer’s test without anesthesia in field area. The data was recorded separately for age and sex groups. The DED determination was done on the basis of positive Shirmer’s test reading less than 10 mm. There were three age groups 0 to 25 years, 25 to 50 years and more than 50 years. Male and female were recorded separately. Results: Prevalence of DED in females 0-25 year group 5.5%, 25-50 years 15.1% and more than 50 years 57.01%. Males had 0-25 years group 4.1%, 25-50 years 15.9% while more than 50 years 65.1%. Overall prevalence for 0-25 years 4.77%, 25-50 years 15.5% and more than 50 years was 60.87%. The DED prevalence in rural population was determined 19.2% which is in the same order with previous studies. Conclusion: However more resources required conducting other diagnostic test for DED in field settings, then the prevalence may be more in rural population as accepted theoretically.