Background: To compare the outcome of osteoarthritis of knee managed by intra-articular platelet rich plasma versus prolotherapy. Materials & methods: 40 Patients were selected from orthopaedics OPD after taking written informed consent. The patients were divided into 2 groups as follows: Intra-articular Platelet Rich Plasma group and Intra-articular prolotherapy injection. Based on the WOMAC score, clinical outcome analyses were performed to determine the effectiveness. Using 24 characteristics, the WOMAC (Western Ontario and McMaster Universities) score was utilised to evaluate individuals with hip or knee osteoarthritis. All the results were recorded and analyzed using SPSS software. Student t test and ANOVA test were used for evaluation of level of significance. Results: At 5 weeks follow-up, among the patients of Intra-articular Platelet Rich Plasma group, mean functional limitation score, pain level score, stiffness score and overall WOMAC score was 28.1, 9.2, 3.6 and 40.9 respectively. Among the patients of Intra-articular prolotherapy injection group, mean functional limitation score, pain level score, stiffness score and overall WOMAC score at 5 weeks was 24.3, 6.2, 3.3 and 33.8 respectively. Significant better results were obtained in terms of WOMAC score at 5 weeks follow-up among the patients of Intra-articular Platelet Rich Plasma group in comparison to Intra-articular prolotherapy injection group. Conclusion: In patients with knee OA, prolotherapy and PRP both considerably improved the outcome in terms of pain, stiffness, and functional restriction. However, PRP injection was more efficient than prolotherapy at reducing pain, stiffness, and functional limitations in patients with knee osteoarthritis.