Background: The occurrence of late-onset neonatal sepsis (LONS) continues to be a major cause of infant morbidity and mortality even with notable advancements in neonatal intensive care. distinct geographical areas have distinct causal organisms and antibiotic susceptibilities, which influences the use of empirical antibiotics in suspected cases of sepsis. Aims and Objectives: To determine the drug sensitivity pattern of the commonly isolated pathogens in LONS. Materials and Methods: This study was done after obtaining approval from the Institutional Human Ethical Committee of Ballari Medical College and Research center. The blood culture sensitivity data of all neonates admitted and treated as cases of LONS over a period of 3 months (December 2013 to December 2015) at the neonatal intensive care unit of Bapuji Hospital were studied retrospectively. Results: A total of 212 neonates admitted were suspected cases of LONS. 45.45% of them were blood culture positive, with 77% of the isolated organisms being Gram-positive and 6(23%) Gram-negative. The most frequently isolated organism was coagulase-negative Staphylococcus aureus (77%) followed by Klebsiellapneumoniae (7%). 71.25% of these organisms showed resistance to ampicillin, and 56.25% showed resistance to gentamicin which is the World Health Organization recommended an empirical choice of antibiotics. Conclusion: According to our research, there is a growing trend of resistance to first-line empirical antibiotics that are frequently used, like gentamicin and ampicillin. Therefore, it is crucial to regularly monitor antibiotic susceptibility in order to select the appropriate empirical antibiotic.