Background: Due to paucity of voluntary body donation practice in our society, the Indian medical institutes have inadequate cadavers for dissection. This research aimed to assess the knowledge and attitude of medical students towards body donation after death and to understand various factors which significantly hinders voluntary body donation. In depth knowledge of medical students with a positive mindset can play a significant role in motivation of general public towards body donation after death. Methods: A cross sectional study was conducted among 200 first year MBBS students of Chirayu Medical College and Hospital, Bhopal, (M.P). A paper based validated questionnaire was used to assess knowledge and attitude of medical students. The data were analyzed statistically and expressed as percent of total study population. Results: 90.5% of medical students were aware about voluntary body donation. None of the participants were sure about documents and legal aspects. 89% students were unwilling to pledge their body for donation. Females were not willing to donate due to fear of disfigurment (19.7%). Dissection hall experience was noted to be main reason for unwillingness (40.5%). Body donation programme was believed to be important facilitator by 49.5% of participants. Conclusions: This Study revealed that medical students were unwilling to pledge for body donation and experience of dissection hall was significant to affect student’s attitude. Also students need more detailed knowledge on all the fundamental and legal aspects of voluntary body donation through awareness sessions so that they can provide correct information to others.