Background: This study was conducted to assess serum lipid profile in subjects having Oral Squamous Cell Carcinoma (OSCC). Material and methods: The study comprised a total of 100 participants. This research included individuals diagnosed with oral cancer through histological confirmation, as well as those without any significant systemic illnesses. Participants who were classified as overweight or aged over 70 years were excluded from the study. The subjects were divided into two groups, each consisting of 50 individuals: one group represented those with oral cancer, while the other comprised healthy individuals. Group 1 consisted of 50 participants without the condition, and Group 2 included 50 individuals diagnosed with the disease. Descriptive statistics were utilized to derive the findings of this study. Categorical variable results are presented as percentages, while continuous variable outcomes are reported as mean ± standard deviation (minimum-maximum). A significance level of five percent was established for the results. To compare research parameters across three or more patient groups, analysis of variance was conducted, with Tukey's post hoc test employed for pairwise comparisons. The 3x3 Fisher exact test was applied to evaluate the significance of categorical study parameters across two or more groups, with a significance threshold set at the 5% level. Results: The investigation indicated that individuals diagnosed with oral cancer exhibited mean plasma triglyceride levels of 1105.4 mg/dl, in contrast to the control group, which had mean levels of 153.6 mg/dl. It was noted that the malignant cohorts presented lower mean plasma triglyceride concentrations compared to the control group. Furthermore, the mean total cholesterol (TC) level was recorded at 122.9 mg/dl for the cancer group, while the non-cancer group had a mean of 212.8 mg/dl. Both the precancerous and cancerous groups demonstrated significantly reduced plasma TC levels relative to the control group. The average high-density lipoprotein (HDL) levels were found to be 81.5 mg/dl in the non-cancer group, whereas the cancer group had an average of 39.6 mg/dl, indicating a notable decline in plasma HDL levels between the precancerous and cancerous groups compared to the control group. Mean plasma low-density lipoprotein (LDL) levels were 111.1 mg/dl for the non-cancer group and 63.4 mg/dl for the cancer group, with both the precancerous and cancerous groups exhibiting significantly lower plasma LDL levels than the control group. The study also revealed that the average plasma very low-density lipoprotein (VLDL) levels were 45.2 mg/dl in the control group and 9.61 mg/dl in the oral cancer group, with both malignant groups showing markedly lower plasma VLDL levels compared to the control group. Additionally, the ratio of total plasma cholesterol to HDL was calculated to be 3.14 in the healthy group and 6.87 in the oral cancer group, indicating a significantly higher cholesterol to HDL ratio in the precancerous and cancerous groups compared to the control group. Conclusion: The association between oral cancer risk and serum lipid profile seems to be adversely associated. Evidence suggests that a decrease in blood lipid levels may act as an early marker of neoplastic cellular changes before clinical symptoms appear.