Background: The key element in laparoscopic surgery is creation of pneumoperitoneum and carbon dioxide is commonly used for insufflation. The present study was conducted to assess the effect of low dose dexmedetomidine infusion on hemodynamic response in patients undergoing laparoscopic cholecystectomy. Materials & Methods: 120 patients were randomly allocated into two groups, 60 patients in each group, Group A (patients receiving normal saline 0.9% infusion) & group B (patients receiving dexmedetomidine infusion at the rate of 0.4 mcg/kg/hour). Results: There was no significant difference in mean BMI, systolic blood pressure, diastolic blood pressure, heart rate, MAP and SpO2 at baseline between Group A and Group B. The mean heart rate at 1 minute after induction, after laryngoscopy & intubation, after pneumoperitoneum, after release of pneumoperitoneum and after 1 min of extubation was significantly more among Group A and Group B. There was no significant difference in distribution of males and females between Group A and Group B. Ramsay Sedation Score 5 1 minute after extubation and 15 minutes after extubation was significantly more among Group B. Ramsay Sedation Score at 60 mins after extubation and score 3 at 120 minutes after extubation was significantly more among Group B. The mean duration of surgery was significantly more among Group A as compared to Group B. Conclusion: There was attenuation of hemodynamic changes during laryngoscopy, endotracheal intubation, pneumoperitoneum and extubation during laparoscopic cholecystectomy surgeries with infusion of Dexmedetomidine at the dose of 0.4 µg/kg/hr.