Introduction: Mycotic Keratitis is one of the difficult forms of keratitis to diagnose and treat successfully. It is important ocular infection, especially in young male outdoor workers with history of vegetative trauma to eye. In this study patients of fungal keratitis were treated with voriconazole drug with topical eye drops versus intrastromal injections and the results were compared. Aim: To compare the efficacy of intrastromal voriconazole versus topical voriconazole in fungal keratitis. Material and method: This is a prospective study conducted on 100 cases of fungal corneal ulcers presented to out-patient department / in-patient department. Cases were diagnosed clinically and confirmed on KOH wet mount preparation, divided into two groups with 50 cases treated with intrastromal VCZ (groupI) and 50 cases treated with topical VCZ (groupII). The healing response results are compared in both the groups. Results: Out of 100 cases, 73% were males with maximum incidence (67%) in age group of 31-60 years. 69% cases were from rural background, agriculturist (73%) by occupation, majority of cases (87%) had history of vegetative trauma causing fungal ulcer. On treatment the healing response in intrastromal VCZ (group I) and topical VCZ (group II) was statistically significant (p <0.05%) that is earlier healing was seen in intrastromal VCZ treated group. On comparison the best corrected visual outcome at 12 weeks, the difference between two groups was insignificant (p >0.05%). Fungal ulcers were healed with corneal opacity. Conclusion: Our data suggests that the voriconazole drug was beneficial in treatment of fungal keratitis and early healing of ulcer was seen with intrastromal voriconazole treatment than topical VCZ treatment. Abbreviations: VCZ: voriconazole, KOH: potassium hydroxide, SDA media: sabourouds dextrose agar media