Klebsiella species isan emerging concern for the worldwide as it is one of the major causes of drug resistant. In infection with Klebsiella species, the fluoroquinolones are considered safe and adequate therapeutic option but several studies reported an increasing percentage resistant to these antimicrobials due to efflux pump activity which made these strains very difficult to treat.Objective of the study is to study the efflux pump activity in ciprofloxacin resistant isolates of Klebsiella species.A prospective and experimental study was carried out over a period of three years. A total of 200 isolates of Klebsiella species isolated from various clinical samples and confirmed by conventional biochemical tests. Antimicrobial susceptibility testing was done as per CLSI 2010 guidelines to determine ciprofloxacin resistance. The resistant strains were subjected to detection of efflux pump by inhibition of efflux pump activity with the use of Carbonyl cyanide 3-chlorophenylhydrazone (CCCP). Minimum inhibitory concentrations (MICs) of all resistant strains to ciprofloxacin were measured with and without use of CCCP.71 out of 200 isolates of Klebsiella species were resistant to ciprofloxacin. Among these 71 isolates; 31 (43.66%) showed 2-32 fold reduction in the MICs value after use of CCCP as an efflux pump inhibitor. The other 40 remaining strains showed no reduction in their MICs value before or after use of CCCP.Efflux pump has a crucial role and it is one of the major causes of ciprofloxacin resistant in Klebsiella species. Routinely detecting the efflux pump and restricting the use of ciprofloxacin in such strains will certainly limits the development of further resistance as well as it also help clinicians to prescribe proper antibiotics to manage infections.