Background:Among the most prevalent illnesses in humans are UTIs. The present study was conducted to evaluate Fosfomycin sensitivity pattern against uropathogens. Materials & Methods:58 patients with UTI of both genders were selected. Midstream clean catch urine samples were collected in a sterile urine container. The significant growths of pathogenic bacteria were subjected to antibiotic susceptibility testing. Fosfomycin (200 µg) disc was used in Kirby-Bauer disc diffusion testing. Fosfomycin trometamol MIC was determined by agar dilution method Results: Out of 58 patients, 20 were males and 38 were females. Organism isolated were E. coli in 42, Enterococcus spp. in 8, Klebsiella spp. in 2, Proteus spp. in 1, Enterobacter spp. in 2, Pseudomonas spp. in 2, and Acinetobacter spp. in 1 case. The difference was significant (P< 0.05). E. coli showed maximum susceptibility against FOS (40) followed by NIT (38). Enterococcus spp. showed maximum susceptibility against FOS (7) followed by CZ (6). Klebsiella spp. showed maximum susceptibility against FOS (2), and FQ (2). Proteus spp. showed maximum susceptibility against FOS (1), FQ (1), COT (1) and CZ (1). Pseudomonas spp. showed maximum susceptibility against FOS (1), and Acinetobacter spp. showed maximum susceptibility against showed maximum susceptibility against NIT (1), COT (1) and CZ (1). Conclusion: Fosfomycin trometamol's high susceptibility and low MIC distribution imply that it should be used in conjunction with nitrofurantoin as part of a patient's empirical therapy arsenal for UTIs.