Introduction:- Infection of the eye leads to conjunctivitis, keratitis, endophthalmitis, dacryocystitis, blephritis, infections of eye lid, microbial scleritis, canaliculitis, preseptal cellulitis, orbital cellulitis, endophthalmitis and panophthalmitis etc., which are responsible for increased incidence of morbidity and blindness worldwide. Aim:- To study the microbiological profile of ophthalmic infections and the antibiotic susceptibility pattern of the bacterial isolates at a tertiary care hospital, Kanpur. Materials and methods:- A Total 50 samples were received from infections of the eye- including conjunctivitis, corneal ulcers; cataract and FB cornea infections. The samples were processed on Blood agar and MacConkey agar, incubated aerobically at 370C for 24 hours. Samples from ophthalmic infections cases were aerobically cultured and isolates from culture positives were identified by standard procedures. Antimicrobial susceptibility testing was done following CLSI guidelines 2020. Result:- Out of 50 cases 34(34% ) were positive for bacterial growth. Predominant bacterial isolates S. aureus 22(64.70%) S.lugdunensis 7(20.55%) MRSA 3(8.82%) CoNS, 1(2.94%),and MR CoNS 1(2.94%), were isolated Gram Positive Bacteria. All isolates were susceptible to Amikacin, Gentamicin, Linezolid, Teicoplanin, Tetracycline, Tobramycin. Frequency of inducible clindamycin resistance among Staphylococcus aureus was 7(31.81%), Staphylococcus lugdunensis was 3(42.85), Methicillin resistant Staphylococcus aureus was 1(33.33%),MR CoNS was 1(100%) and among CoNS it was 1(100%). Conclusion: Our findings are in general consistent with those from clinical studies of Ophthalmic infection. The most commonly isolated bacterial pathogens in this study were gram positive cocci. S. aureus and S. lugdunensis are the most common bacterial isolates found in the conjunctivitis infection. Amikacin, Gentamicin, Linezolid, Teicoplanin, Tetracycline Tobramycin, Levofloxacin and Vancomycin showed the lowest resistance rates to all bacterial isolates.