Background: Post-operative wound infection or surgical site infection (SSI) is a significant cause of nosocomial infection among patients who have undergone surgery. The present study was conducted to assess prevalence of Pseudomonas aeruginosa and its antimicrobial sensitivity profile among postoperative wound infections. Materials & Methods: 90 post operative wound swabs of both genderswere processed according to the standard microbiological techniques. The identification of bacterial isolates was done by standard biochemical and automated techniques. Antimicrobial Susceptibility Testing (AST) of the isolates was performed on Mueller Hinton agar by Kirby Bauer’s disc diffusion method. Results: P. aeruginosa was seen in 32, Escherichia coli in 20, Klebsiella pneumoniae in 12, Staphylococcus aureus in 10 and Proteus mirabilis in 16 cases. The difference was significant (P< 0.05). Out of 20 diabetic foot specimens, P. aeruginosa was isolated in 6 cases, out of 36, abscess drainage, 20 had P. aeruginosa, out of 198 CS, 4 had P. aeruginosa, out of 10 bone excision, 2 had P. aeruginosa. The difference was significant (P< 0.05).Amikacin was sensitive in 85%, Aztreonam in 81%, Cefepime in 76%, Ceftazidime in 75%, Ciprofloxacin in 82% and Meropenem in 76%. Conclusion: There was high prevalence of Pseudomonas aeruginosa among postoperative wound infections. Antibiotic sensitivity profile of P. aeruginosa was maximum with Amikacin.