Introduction: Breast cancer remains a significant global health challenge and a leading cause of female mortality, prompting ongoing exploration for novel biomarkers and therapeutic options. Prostate-specific antigens (PSA) have been identified in female tissues regulated by steroid hormones, with trace amounts consistently present in female serum. Methods: This observational study involved 128 individuals admitted to surgical and oncology wards. Serum PSA levels were analysed preoperatively and postoperatively in 64 breast cancer patients and 64 controls, including premenopausal and postmenopausal individuals, and distinguishing between early and locally advancedbreast carcinoma. Results: Preoperative PSA levels in breast cancer patients (0.273+/- 1.80) did not significantly differ from controls (0.051+/_ 0.058, p=0.328). No significant PSA level variations were observed among age groups or menopausal statuses within the study or control groups. Comparison of pre- and postoperative PSA levels within breast cancer patients revealed no significant differences (p=0.311). Discussion: Although previous research suggests PSA's potential as a prognostic indicator, this study found no substantial correlation between serum PSA levels and breast cancer diagnosis, both preoperatively and post- operatively. Larger scale, randomised trials are needed to determine its diagnostic significance in female serum.