Background: The COVID-19 (Coronavirus disease 2019) pandemic led to closure of educational institutions worldwide that significantly disrupted the traditional class room based educational system necessitating a shift to e-learning modalities. The aim of our study was to assess the perception of e-learning among the medical, dental and nursing students of a Medical college in Southern Assam during the COVID-19 pandemic. Methods: A questionnaire-based survey using Google Forms was conducted forsix months among the MBBS (Bachelor of Medicine and Bachelor of Surgery), BDS (Bachelor of Dental Surgery) and BSc (Bachelor of Science) Nursing students of the Institution. A total of 306 students consented to participate in the study. Results: Out of the 306 students, 54.9% were BSc Nursing, 42.2% were MBBS of Phase-2 & Phase-3 (Part-1), and 2.9% were BDS students. The majority (60.1%) of the students had not participated in any form of e-learning before COVID-19. While 59.2% regularly searched online resources, 72.9% did not prefer online teaching over classroom instruction and missed the classroom ambience. Approximately one-third (35.3%) believed e-learning leads to social isolation, and 51.3% felt the lack of face-to-face interaction reduced learning effectiveness. However, 36.6% reported of being disturbed by others during online sessions. Nearly one-third (28.4%) suggested continuation of a part of online sessions post-pandemic. Conclusion: E-learning was a valuable teaching method that enhanced knowledge during the pandemic. However, students highlighted concerns like social isolation, reduced interaction, and distractions. A blended approach combining e-learning with face-to-face classroom instruction may be an effective post-pandemic educational strategy that requires a more active approach.