Background: The middle ear cleft's chronic inflammation in the presence of a persistent tympanic membrane perforation is known as chronic otitis media - mucosal type. The present study was conducted to compare the clinical outcomes of type I tympanoplasty done with and without fibrin glue. Materials & Methods: 60 patients with tympanic membrane perforation of both genders were divided into 2 groups. In group I, fibrin glue was applied along the edges of the perforation. In group II, fibrin glue was not applied. Parameters such as side, type, hearing improvement, and outcome was recorded on recall visits. Results: Out of 60 patients, 32 were males and 28 were females. Side was right in 16 and 13 and left in 14 and 17 in group I and group II respectively. Quadrants were antero-inferior in 9 and 9, antero-superior in 10 and 6, postero-inferior in 6 and 8 and postero- superior in 5 and 7 patients respectively. Pure tone average pre-operative value was 34.6 and 37.4, at 3 months was 25.3 and 24.5 and at 36 months was 23.9 and 22.1 in group I and group II respectively. The difference was significant (P< 0.05). Graft uptake rate in group I was 95% and in group II was 92%. Conclusion: Fibrin is safe to use in middle ear procedures and there is no need to worry about negative side effects. Fibrin glue is too expensive to be included in the conventional type I tympanoplasty treatment, but it can be used to secure the graft in patients with extensive perforations, therefore individuals with subtotal perforations may want to consider it.