Background:The femur represents the longest, heaviest, and most robust bone in the human skeletal system. Femoral rotational asymmetry (FRA) refers to an anatomical discrepancy where the posterior condyles and the trochlear groove lack perpendicular alignment. Hence, the present retrospective study was conducted for evaluation of the femoral rotational asymmetry Materials &Methods:50 patients were scanned resulting in 100 knee scans. The deepest locations within the trochlear groove, indicative of the sulcus line, were designated as regions of interest (ROIs) on the surface rendering and validated through orthogonal views in a multiplanar reconstruction format. The sulcus line (SL) was then quantified in relation to the surface area of the epiphysis (SEA) and the posterior condyle (PC) employing the “angle” tool, with the resulting data recorded in a Microsoft Excel spreadsheet. The ROI points were inserted. All the results were recorded and analyzed using SPSS software. Results:The mean WL and SL were +2.7° and +1.1° respectively. The mean PC was -1.9°.In 58 percent of the cases, the rotational landmark was WL while it was SL in 25 percent of the cases.The Average SL and PC + 3° and PCL (Kinematic alignment)was present in 18 percent and 35 percent respectively. Conclusion:Both the SL technique and the WL technique can be employed to assess the rotational alignment of the trochlear groove with SL showing slightly better results.