Aim: To study the prevalence of Uric Acid as risk factor associated with severity of NAFLD. Objectives: Primary objective: To assess prevalence of uric acid in NAFLD patients. Secondary objective: To assess association of uric acid as risk factor in NAFLD patients. Methodology: Present study was an observational prospective study between January 2023 to June 2024 in patients between age group 25-65 years attend OPD/IPD services at Department of Medicine, Mahatma Gandhi Hospital, Jaipur. Results: In this comparative analysis of hyperuricemia prevalence between individuals with and without non-alcoholic fatty liver disease (NAFLD), significant differences were observed. Among the 160 participants without NAFLD, 18 individuals (11.2%) were identified as having hyperuricemia. In contrast, among the 160 participants diagnosed with NAFLD, a notably higher proportion, comprising 55 individuals (34.4%), exhibited hyperuricemia. Statistical analysis revealed a highly significant p-value of less than 0.0001, indicating a strong association between the presence of NAFLD and the prevalence of hyperuricemia. Conclusion: The association between uric acid levels and non-alcoholic fatty liver disease (NAFLD) has been a subject of growing interest in recent years. In current study, elevated uric acid levels were significantly associated with NAFLD (p < 0.0001), supporting the link between hyperuricemia and NAFLD (Zhu et al., 2014).